Development and validation of a new method for determination of topiramate in bulk and pharmaceutical formulation using high performance liquid chromatography UV detection after pre column derivatization

Mohammadbagher Majnooni; Ronak Jalili; Bahareh Mohammadi; Shahram Miraghaee; Mohammad Taher Bahrami; Sajad Fakhri; Gholamreza Bahrami

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Development and validation of a new method for determination of topiramate in bulk and pharmaceutical formulation using high performance liquid chromatography UV detection after pre column derivatization

Author(s):

Mohammadbagher Majnooni¹,

Ronak Jalili^2,*,

Bahareh Mohammadi²,

Shahram Miraghaee²,

Mohammad Taher Bahrami²,

Sajad Fakhri

²,

Gholamreza Bahrami^{1, 2}

1Department of Pharmacology and Toxicology, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

2Medical Biology Research Center, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 3, issue 2; 179-183

Published online:Nov 15, 2014

Article type:Research Article

Received:Apr 02, 2014

Accepted:Oct 06, 2014

How to Cite:Mohammadbagher MajnooniRonak JaliliBahareh MohammadiShahram MiraghaeeMohammad Taher BahramiSajad FakhriGholamreza Bahramiet al.Development and validation of a new method for determination of topiramate in bulk and pharmaceutical formulation using high performance liquid chromatography UV detection after pre column derivatization.J Rep Pharm Sci.3(2):e147711.

Abstract

In published high performance liquid chromatographic (HPLC) methods for analysis of topiramate (TPM) in pharmaceutical dosage forms and raw materials Refractive Index Detector (RID) has been used which is not available in many laboratories and has low sensitivity. We described a new, sensitive and simple HPLC method for determination of topiramate in pharmaceutical forms and Invirto dissolution studies which avoids the use of RID detector. The method is based on derivatization of topiramate and an internal standard by reaction with 4‐chloro‐7‐nitrobenzofurazan (NBD‐CL), and reverse‐phase chromatography using phenyl column and spectrophotometer detection at 264 nm. A mixture of phosphate buffer (0.05 M) containing triethylamine (0.1٪ V/V; pH=2.3) and methanol (28:72, V/V) at a flow rate of 2.2 ml/min was used as mobile phase. The analysis performance was studied and the method was shown to be selective and linear for determination of topiramate in pharmaceutical forms and dissolution studies.

Keywords

Topiramate

Pharmaceutical Formulation

Derivatization

HPLC

Dissolution Study

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