A Novel, Efficient, Fast and Inexpensive DNA Extraction Protocol from Whole Blood Applicable for Studying Drug-DNA Interaction

Mohammad Taher Moradi; Kheirollah Yari; Reza Khodarahmi

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A Novel, Efficient, Fast and Inexpensive DNA Extraction Protocol from Whole Blood Applicable for Studying Drug-DNA Interaction

Author(s):

Mohammad Taher Moradi¹,

Kheirollah Yari

^2,*,

Reza Khodarahmi¹

1Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

2Student Research Committee, Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 3, issue 1; 80-84

Published online:Apr 13, 2014

Article type:Research Article

Received:Jan 27, 2014

Accepted:Mar 27, 2014

How to Cite:Moradi M T, Yari K, Khodarahmi R. A Novel, Efficient, Fast and Inexpensive DNA Extraction Protocol from Whole Blood Applicable for Studying Drug-DNA Interaction.J Rep Pharm Sci.2014;3(1):e147729.

Abstract

The DNA molecule has been known to be the cellular target for many cytotoxic anticancer agents for several decades. Understanding how drug molecules interact with DNA has become an active research area in the interface between chemistry, molecular biology and medicine. DNA extraction has been suggested as a main step affecting molecular DNA technology such as PCR and PCR-based methods. Therefore, researchers have used several modified protocols for efficient DNA extraction from whole blood. In this study, we focused on a fast and reliable protocol with inexpensive and non-poisonous reagents for DNA extraction from whole blood. Current method was optimized based on a combination of conventional salting-out and boiling methods. Also the quality and quantity of the extracted DNA were surveyed by gel electrophoresis and Nanodrop spectrophotometry methods, respectively. Results showed that high quantity and quality of isolated DNA by this method is enough to do hundreds of PCR-based reactions and also to be utilized in other DNA manipulation assay such as restriction digestion, drug- DNA interaction and methylation detection survey. In conclusion, we described a fast, low-cost, non-toxic and enzyme free protocol for high yield genomic DNA extraction from whole blood.

Keywords

DNA extraction

Whole blood

Drug-DNA interaction

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