Dispersive Liquid-Liquid Micro-Extraction as a Sample Preparation Method for Clonazepam Analysis in Water Samples and Pharmaceutical Preparations

Loghman Miri; Fahimeh Jalali

Outlines

Abstract
Copyright

Dispersive Liquid-Liquid Micro-Extraction as a Sample Preparation Method for Clonazepam Analysis in Water Samples and Pharmaceutical Preparations

Author(s):

Loghman Miri¹,

Fahimeh Jalali^1,*

1Department of Chemistry, Razi University, Kermanshah, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 2, issue 2; 103-110

Published online:Jun 08, 2013

Article type:Research Article

Received:May 10, 2013

Accepted:May 27, 2013

How to Cite:Loghman MiriFahimeh JalaliDispersive Liquid-Liquid Micro-Extraction as a Sample Preparation Method for Clonazepam Analysis in Water Samples and Pharmaceutical Preparations.J Rep Pharm Sci.2(2):e147739.

Abstract

Dispersive liquid–liquid micro–extraction (DLLME) technique was successfully used as a sample preparation method for the determination of clonazepam in pharmaceutical preparations and water samples. In this method, a suitable mixture of methanol (disperser solvent) and chloroform (extraction solvent) was injected rapidly into a conical test tube that contained an aqueous solution of clonazepam. After centrifuging, phase separation was performed by sedimenting the fine droplets of the micro– extraction solvent on the bottom of a test tube (about 100 μL) and then the absorbance of the enriched extracted phase was determined at the absorption wavelength of clonazepam (307 nm). Some important parameters such as, the type and volume of extraction and dispersive solvents as well as the extraction time were investigated and optimized. Under the optimum conditions, the calibration graph was linear over the range of 0.95 to 18.94 μg/mL of clonazepam with a limit of detection of 0.06 μg/ml.

Keywords

comments