The Antiproliferative Effects of Petroleum Ether Extract of Artemisia Aucheri on Human Cancerous Cell Lines

Mahmoud Ghazi-Khansari; Mahdi Mojarrab; Farahnaz Ahmadi; Leila Hosseinzadeh

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Abstract
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The Antiproliferative Effects of Petroleum Ether Extract of Artemisia Aucheri on Human Cancerous Cell Lines

Author(s):

Mahmoud Ghazi-Khansari¹,

Mahdi Mojarrab²,

Farahnaz Ahmadi¹,

Leila Hosseinzadeh^2,*

1Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

2Novel Drug Delivery Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 2, issue 2; 150-155

Published online:Oct 02, 2013

Article type:Research Article

Received:Sep 13, 2013

Accepted:Sep 26, 2013

How to Cite:Mahmoud Ghazi-KhansariMahdi MojarrabFarahnaz AhmadiLeila HosseinzadehThe Antiproliferative Effects of Petroleum Ether Extract of Artemisia Aucheri on Human Cancerous Cell Lines.J Rep Pharm Sci.2(2):e147748.

Abstract

Different species of Artemisia (Asteraceae) have shown to exhibit antitumor activity. The aim of this study was to identify the anti-proliferative effect of Artemisia aucheri Boiss (A.aucheri) from Iran on cultured human cancer cells. Aerial parts of A. aucheri Boiss. were collected from Chahar Bagh region , Golestan province, Iran. The dried powdered aerial parts of A.aucheri were extracted with petroleum ether (40-60), dichloromethane, ethyl acetate, ethanol and ethanol-water (1:1 v/v), respectively. For biological investigation different concentrations of extracts were added to cultured cells and incubated for 24 h. MTT assay was employed to assess the cell viability. In addition, morphological changes of cells were assayed using phase contrast inverted microscope. Different extracts exert various growth inhibitory effects. In case of SKNMC, MCF-7 and A2780 cell lines, petroleum ether extract (IC50 values: 21.0, 26.0 and 28.0 μg/ml, respectively) showed the highest growth inhibitory effects. Dichloromethane extract showed IC50 values: 27.0, 35.0 and 55.0 μg/ml, respectively.This study showed the anti-proliferative effects of A.aucheri petroleum ether extract on malignant cell lines. Thus, the aforementioned may be considered as a promising chemotherapeutic agent in cancer treatment.

Keywords

Artemisia aucheri

Extract

Cytotoxiciy

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