Abstract
H-point standard addition method (HPSAM) with simultaneous addition of both analytes in nonaqueous solution was applied for determination of levonorgestrel (LEV) and ethinyl estradiol (EE) in LD and HD tablets. The results showed that, simultaneous determination could be performed with the ratio 1 to 5 of LEV-EE. The LOD for LEV and EE were obtained 5.1×10-7 and 9.6×10-7 M, respectively, and the corresponding values of %RSD (n=4) for LEV and EE were 1.96 and 2.69, respectively. Underworking conditions, the proposed method was successfully applied for simultaneous determination of LEV and EE in several synthetic mixtures and LD and HD tablets.
Keywords
H-Point Standard Addition Method Levonorgestrel Ethinyl Estradiol LD And HD Tablets HPLC
Copyright
© 2012, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.