The present study aims to determine the effect of three different volumes of dynamic stretching on linear repeated-sprint performance over thirty-meters in team-sport athletes. The main finding of this study showed that performing two sets of dynamic stretching (DSS2), incorporating five active dynamic exercises (lower body musculature), significantly improves linear repeated sprint performance compared to one set (DSS1) or three sets (DSS3), respectively. It has long been established that performing a warm-up prior to competition is crucial to maximizing and optimizing performance (
2,
3,
9). Many research studies looking at the effect of various active warm-up protocols, that include stretching (such as passive, active, PNF and ballistic), on athletic performance have been conducted. However, the literature does not provide a clear consensus regarding the effects of stretching on performance and findings are often contradictory (
9,
25). Although findings in the literature are confounding, completing an active warm-up prior to physical activity has a positive impact on the subsequent exercise performed if it has been structured appropriately (
2,
3). A lot of available evidence suggests that incorporating dynamic stretching within the warm-up positively influences numerous aspects of athletic performance (
6,
12,
19,
23), as opposed to other forms of stretching (
36,
44). However, aspects such as the warm-up intensity, the specificity of the warm-up, the duration of the warm-up and the duration of recovery all appear to affect whether optimal performance can be achieved through dynamic stretching (
3-
5,
9).
Previous findings have tended to show improvements in singular sprint performance following dynamic stretching compared to passive stretching or no stretching or a combination of both (
20,
24,
25). It has been found that performing static or passive stretching has a detrimental effect on singular sprints due to a decrease in neural transmission (
20) and/or insufficient increase in muscle and/or rectal temperature pre-exercise (
2,
3). When looking at repeated-sprint performance similar findings can be observed. Static stretching on its own or in combination with dynamic stretching negatively impairs repeated-sprint performance (
10,
36,
45). However, some studies have found this not to be the case (
37,
46). The large methodological variations in repeated-sprint protocols, warm-up strategies and training status of subjects make comparisons between studies in the literature difficult. Considering many studies find repeated-sprint performance to be positively associated with dynamic warm-up, there is a need to determine the most effective volume of dynamic stretching on subsequent performance to make valid comparisons and provide more detailed information for practitioners and coaches.
Findings in the literature have shown that 1 or 2 sets of dynamic stretching improved 20-m sprint performance compared to 3 sets, but no differences were found for 10-m sprint performance (
25). When looking at repeated-sprint performance, prior activity highly influences the ability to perform repeated sprints because of impaired rates of muscle power output ability and its association with neural adjustments (
29). A study performed by Ishak et al. (
8) found no differences in repeated-sprint performance over 20-m when performing a volume of 1, 2 or 3 sets of dynamic stretching. Our study found that linear repeated-sprint performance in team-sport athletes consisting of 6 maximal 30-m sprints with 30s of active recovery, is affected by the volume of dynamic stretching performed in the warm-up. We established a decrease of 4.1% following 1 set of dynamic stretching and 2.8% following 3 sets of dynamic stretching in AST and TST compared to 2 sets of dynamic stretching. Our outcomes therefore suggest that to improve or achieve optimal linear repeated-sprint performance over 30-m, it is advised to structure a warm-up incorporating 2 sets of dynamic stretching prior to activity. A single set protocol yielded the most negative findings and may not have provided enough stimulus for recovery or increased rectal and/or muscle temperatures to an adequate level prior to linear repeated-sprint performance (
30). In addition, performing 3 sets of dynamic stretching also reported linear repeated-sprint performance to be significantly worse compared to 2 sets and is deemed to be too intense as a warm-up procedure prior to this mode of exercise. Interestingly, no differences were established between any of the conditions when comparing BST (P > 0.05).
Furthermore, fatigue responses also varied between conditions. Fatigue indexes, a concept which has previously been used to investigate the development of fatigue during repeated-sprint performance, varied across the different conditions. The higher the FI, the lower an individual’s ability to maintain power over a series of sprints. It was found that DSS2 displayed the lowest FI when compared to DSS1 and DSS3 (P < 0.05). Our athletes were unable to repeat the same maximum power outputs after DSS1 and DSS3. We believe that not enough/too much stimulus was provided for recovery or adequate levels of rectal and/or muscle temperatures were reached prior to the repeated-sprint performance (
38). DSS3 was deemed to be too intense as a warm-up. This potentially resulted in a decrease in the availability of high-energy phosphates and resulted in reduced contraction velocity capability of the muscles involved (
40). A longer rest period prior to repeated-sprint performance test would be necessary to establish whether DSS3 could result in an increase in repeated sprints, to allow for the re-synthesis of high energy phosphates, while ensuring muscle temperature and rectal temperature is still elevated (
47). It can be suggested that two sets of dynamic stretching resulted in the most “optimal” increase in muscle and rectal temperature, which is closely associated with an increase in the speed of nerve impulses and sensitivity of nerve receptors (
10). However, whether performing DSS2 resulted in muscle contractions to be more rapid and forceful due to higher core and muscle temperatures is unknown. It should further be noted that our specific DSS2 warm-up protocol may not produce the most beneficial physiological and performance changes. We are unable to confirm whether the players commenced the repeated-sprint performance test in a non-fatigued state and with an elevated baseline VO
2, which would result in repeated-sprint performance to further improve. In addition, enhanced repeated-sprint performance after dynamic stretching could also be attributed by an enhanced musculotendinous unit (MTU) stiffness. The gains in flexibility have been primarily attributed to a decrease of musculotendinous unit (MTU) stiffness (
12,
41). The key attributes of dynamic stretching include enhanced motor unit excitability and improved kinesthetic sense, leading to improved proprioception and pre-activation (
42).
We also found heart rate responses and La values to be higher in DSS1 and DSS3. The La values observed post linear repeated-sprint performance were closely related to FI, displaying lower La values in the DSS2 condition. In agreement with previous findings that have also found levels of La > 10 mmol.L
-1 following repeated sprints, it can be suggested that this is associated with inducing muscle deoxygenation and increased ventilatory measures (
38,
39). The marked decrease in repeated-sprint performance during DSS1 and DSS3 is potentially associated with the inability to maintain a high-power output due to a reduction in oxygen availability, is closely associated with a higher accumulation of blood lactate and related to several mechanisms that contribute to fatigue.
Our study provides novel information and shows that if appropriate warm-up prior to repeated-sprint performance is not undertaken, an adverse effect is present. The exact underlying mechanisms responsible for an improvement in performance in repeated-sprint following two sets of dynamic stretching still needs to be thoroughly documented and would provide pertinent information for coaches and athletes. Whether or not our specific dynamic stretching protocol incorporating five active dynamic exercises, related to sprinting by stretching the lower body musculature is the optimal warm-up is unknown and requires more research. Considering no differences were found in BST between the three conditions, this would suggest that this warm-up would not be beneficial for singular sprints. Additionally, performance in team-sports is highly dependent on a myriad of factors such as skill proficiency in addition to physiological and cognitive capabilities. Although it was found that linear repeated-sprint performance was improved, more research is required to gain a better understanding regarding warm-up effects on team sport performance.
It must also be noted that there are limitations to this study. Although a lot of the previous research has used a repeated-sprint protocol with sprints lasting 5 or 6-seconds or 20 to 30-m in distance (
8,
25,
33), recent studies related to field-based team sports have suggested or used sprints 3- seconds in duration or less, or 15-m in distance (
30,
31,
48-
53). Therefore, it would be beneficial to establish whether two sets of dynamic stretching would incorporate the same results over this shorter distance. Further, incorporating other forms of warm-up to compare between different warm-up modes will help provide us with a clearer picture as to which protocol is the most beneficial.
5.1. Conclusions
In conclusion, the results from this study suggest that performing two sets of 5 dynamic stretches of the lower body musculature positively effects linear repeated-sprint performance in team-sport athletes. It could be suggested that increased muscle and rectal temperatures result in an increase in the speed of nerve impulses and sensitivity of nerve receptors and as a result improve linear repeated-sprint performance. Furthermore, two sets of dynamic stretching enhances musculotendinous unit (MTU) stiffness and improves kinesthetic sense, leading to proprioception and pre-activation to be significantly better. Although two sets of dynamic stretching provided beneficial effects on linear repeated-sprint performance, more in-depth research is still required to establish the intensity, duration and recovery period is needed to yield the most positive results.