The main find of the present study was that A) RSASL and RSACOD differed for time variables (TT and BT) and HR responses with better performance for TT and BT in RSASL, and higher mean and peak HR in RSACOD, and B) the percentage differences in TT and BT between the two groups of adolescent athletes decreased from RSASL to RSACOD, although the latter exercise lasted longer. The worst (slower) performance in time variables (TT +38.6% and BT +38.0%) in RSACOD should be attributed to the fact that this exercise protocol included both acceleration and deceleration in order to change direction, whereas RSASL included only acceleration. When differences were examined between basketball players and controls, a trend of worst performance in TT and BT for both RSA exercise protocols in basketball players than in controls was observed. Basketball players were worst in TT and BT in RSASL than in controls (mean difference +1.18 seconds and +0.12 seconds, respectively) and it would be expected that since RSACOD lasted longer the difference between the two groups would increase. Furthermore, adding a COD, i.e. a trajectory with turn of 180 degrees angle would be expected to benefit the shorter group (in the present study, the shorter group was the control group) (
6), since the large anthropometric dimensions of basketball players might not facilitate such turns. Surprisingly, the respective differences in RSACOD were +1.08 seconds and +0.07 seconds), i.e. basketball players reduced their difference from the control group. An explanation for this trend might be the sport specialization, according to which basketball players relatively improved their performance in the tasks with COD, because they use mostly sprints with COD during training and playing. With regards to the average work to rest ratio in the two RSA exercises, it was 1/10 in RSASL and 1/7 in RSACOD. This variation in the work to rest ratio was due to the longer duration of a sprint with COD than of that without COD resulting in shorter rest time in the former than the latter given that in both exercises sprints started every 30 seconds. The different work-to-rest ratio in the two exercises influenced the metabolism, because it has been shown that a shorter rest time between repeated sprints might result in smaller restoration of phosphocreatine stores (
2). Despite the different work-to-rest ratio, the findings of the study did not show any difference in the decrease of performance (FI) between the two RSA exercise protocols. FI was ~ 3.5% for both protocols, which was in agreement with a previous study using a longer RSA protocol (10 × 15 + 15 m with 30 seconds passive recovery, exercise to rest ratio 1/5) (
4) indicating that the rest duration was sufficient in order not to prevent further decreases in performance. The magnitude of the correlations between RSASL and RSACOD with regards to TT and BT was very large. This indicated that the two exercise protocols shared a large portion of variance for TT and BT (71% and 55%, respectively). This portion was larger for TT than for BT. An explanation for this discrepancy was that TT was the total score of exercise, ranging from ~ 27 s in RSASL to ~ 38 s in RSACOD. Since there was maximal effort in both exercise protocols, the large common portion of variance suggested that both protocols taxed similarly the energy transfer systems (i.e. alactic and lactic anaerobic metabolism) and running ability. On the other hand, in the case of BT, i.e. the best single performance, the effect of COD was more intense, because the duration of single trials was ~2.70 seconds in RSASL and ~3.70 seconds in RSACOD. To examine the effect of the two RSA exercise protocols on muscular fatigue, the participants performed a jumping test (CMJ) before and after each protocol. The findings showed an overall main effect of RSA exercise on CMJ, with posttest value being ~2 cm higher than pretest. This finding was not in agreement with previous studies that had used jumping tests (Abalakov test or CMJ) to examine the effect of an RSA test protocol (
14) or small-sided basketball games (
15) on muscular fatigue. In the first study, there was ~ 9% decrease in Abalakov test after an RSA test (6 × 35 m with 10 seconds) (
14). This discrepancy should be attributed to the use of longer distance and shorter rest period compared to the present study. In the second study, CMJ decreased by ~ 2 cm after 3 × 3 game and increased by ~ 3 cm after a 4 × 4 game, which led the authors to conclude that the former game format was more intense and with greater physiological impact than the latter one (
15). In the present study, the pretest CMJ had correlation with TT of larger magnitude in RSACOD than in RSASL, and the same trend was noticed for the correlation of CMJ with BT. This finding showed the additional muscular effort that was needed in the RSACOD. Since the two RSA exercises were novel, there were not any reference data to compare the scores of the participants in the present study. With regards to their jumping performance, CMJ in the basketball players (~ 35 cm) was lower than the values reported for Greek (38.6 cm) (
16), Italian (39.9 cm) (
4) and Tunisian basketball players of similar age (41.4 cm) (
17), and adult basketball players (39.8 cm (
3). The basketball players were taller and heavier than the control group. Stature (184 cm) was similar as that previously reported in 17 years (182 cm) (
16) and in 16 years basketball players of elite Greek clubs (186 cm) (
18), and lower than in 17 years select basketball players from the Dutch basketball academy (Netherlands) (190 cm) (
6). Body mass (76.5 kg) was similar to that of 17 year Dutch basketball players (77.6 kg) (6), and lower than Greek basketball players (79.3 kg) (
18). The larger body dimensions in basketball players than in the control group confirmed previous studies, which highlighted the importance of stature and body mass for performance in basketball (
19,
20). RSA has been shown to be an important component of performance in basketball. Thus, coaches and fitness trainers who work with young basketball players should monitor regularly RSA and include repeated sprints in their training program. The findings of the present study provided reference data on time variables of two RSA exercises, and their impact on HR responses and muscular fatigue, and, consequently they would serve as a valuable tool for coaches and fitness trainers. A limitation of the present study was the small size of the control group. In addition, the control group consisted of athletes competing in different sports. Thus, the findings should be considered with caution taking into account these limitations. The present study focused on repeated sprints. However, considering the pattern of movements in basketball (many changes of directions, not only in forward-backward, but also in lateral directions), future studies should examine the physiological impact of agility protocols and the role of sport specialization.