Image Credit:Shiraz E-Med J
Outline
Development and Evaluation of a Real-Time TaqMan-PCR for the Detection of Human Cytomegalovirus DNA in Bone Marrow Transplant Recipients
Authors
Abstract
Introduction: Cytomegalovirus (CMV) has been recognized as the most important viral pathogen in persons undergoing
bone marrow transplantation (BMT). In this study, we present the development of a TaqMan-based real-time PCR
assay to quantify human cytomegalovirus (CMV) DNA in peripheral blood leukocytes (PBLs) of bone marrow transplantation
patients.
Materials and Methods: A plasmid containing the target sequence from the pp65 region (UL83) of CMV was constructed
as a positive control template. Serial dilutions of 107 to 101 plasmids per assay were prepared. Peripheral blood
samples were collected from patients after transplantation. CMV DNA was quantified by RQ-PCR in parallel with the
pp65 antigenemia assay in PBL samples.
Results: The real-time PCR assay could detect CMV DNA in patient's samples with a wide linear range, from 10 to over
107 copies of CMV. Real-time PCR assay results correlated with those of the CMV pp65 antigenemia assay (P < 0.0001).
Discussion: The TaqMan assay may be a useful tool for rapid quantification of CMV infection and for monitoring of
CMV reactivation in bone marrow transplantation recipients. The results of both quantitative assays were significantly
correlated; however, the RQ-PCR assay was more sensitive than the pp65 antigenemia assay.
Highlights
References
- 1.The references are available in the PDF file.
Copyright
© 2006, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
Detection of Mycobacterium tuberculosis in Peripheral Blood Mononuclear Cells from Patients with Pulmonary Tuberculosis
Yarmohammadi F, Farhadi A, Hassanaghaei N, Rafiei Dehbidi G, Zarghampoor F, et al. Detection of Mycobacterium tuberculosis in Peripheral Blood Mononuclear Cells from Patients with Pulmonary Tuberculosis. Jundishapur J Microbiol. 2023;16(6):e134575. doi: https://doi.org/10.5812/jjm-134575
Development of an In-House TaqMan Real Time RT-PCR Assay to Quantify Hepatitis C Virus RNA in Serum and Peripheral Blood Mononuclear Cells in Patients With Chronic Hepatitis C Virus Infection
Khalvati Fahlyani B, Behzad-Behbahani A, Taghavi SA, Farhadi A, Salehi S, et al. Development of an In-House TaqMan Real Time RT-PCR Assay to Quantify Hepatitis C Virus RNA in Serum and Peripheral Blood Mononuclear Cells in Patients With Chronic Hepatitis C Virus Infection. Hepat Mon. 2015;15(8):e28895. doi: https://doi.org/10.5812/hepatmon.28895
In Vitro Replication of HCV RNA in Peripheral Blood Mononuclear Cells Isolated from Patients Undergoing Treatment for Hepatitis C Virus Infection
Zarnegar G, Farhadi A, Seyyedi N, Aboualizadeh F, Ranjbaran R, et al. In Vitro Replication of HCV RNA in Peripheral Blood Mononuclear Cells Isolated from Patients Undergoing Treatment for Hepatitis C Virus Infection. Hepat Mon. 2020;20(9):e108070. doi: https://doi.org/10.5812/hepatmon.108070
Towards Routine Molecular Diagnostics: Multiplex SYBR Green Real-time PCR for Rapid Molecular Screening of Recurrent Translocations in Acute Myeloid Leukemia
Tavakoli F, Rahmani Seraji H, Roshandel E, Karimpour F, Hasheminasab H, et al. Towards Routine Molecular Diagnostics: Multiplex SYBR Green Real-time PCR for Rapid Molecular Screening of Recurrent Translocations in Acute Myeloid Leukemia. Int J Cancer Manag. 2025;18(1):e166704. doi: https://doi.org/10.5812/ijcm-166704
Evaluation of Cytomegalovirus Infections in Liver Transplant Recipients Under Universal Prophylaxis: A Single Centre Experience
Altunisik Toplu S, Kose A, Karakas S, Bayindir Y, Otlu B, et al. Evaluation of Cytomegalovirus Infections in Liver Transplant Recipients Under Universal Prophylaxis: A Single Centre Experience. Hepat Mon. 2021;21(6):e115370. doi: https://doi.org/10.5812/hepatmon.115370
- Scopus by DOI: 0
Last Update: 3 weeks ago
- Scopus by Title: 0
Last Update: 3 weeks ago
- Scopus by Title (Ref): 0
Last Update: 3 weeks ago
- CrossRef: 0
Last Update: 3 hours ago
- OpenAlex: 0
Last Update: 6 hours ago
- OpenCitations: 0
Last Update: 4 days ago