Retinoic Acid-Oligomeric Chitosan Micelles as Novel Gene Delivery Carrier; in Vitro Transfection Study

Ali Fattahi; Mohammad-Ali Golozar; Jaleh Varshosaz

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Retinoic Acid-Oligomeric Chitosan Micelles as Novel Gene Delivery Carrier; in Vitro Transfection Study

Author(s):

Ali Fattahi^{1, 2, 3,*},

Mohammad-Ali Golozar³,

Jaleh Varshosaz⁴

1Nanosciences and technology research center, Kermanshah University of Medical Sciences, Iran

2Novel Drug Delivery Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah 67346-67149, Iran

3Department of Materials Engineering, Isfahan University of Technology, Isfahan 84146-83111, Iran

4Department of Pharmaceutics, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 2, issue 2; 125-130

Published online:Jul 25, 2013

Article type:Research Article

Received:Jul 02, 2013

Accepted:Jul 20, 2013

How to Cite:Ali FattahiMohammad-Ali GolozarJaleh VarshosazRetinoic Acid-Oligomeric Chitosan Micelles as Novel Gene Delivery Carrier; in Vitro Transfection Study.J Rep Pharm Sci.2(2):e147744.

Abstract

Amphiphilic cationic micelles have recently attracted much attention as non-viral vector. In the current study the retinoic acid-g-chitosan (RA-chitosan) micelles have been used as novel gene carriers. Uptake of micelles, condensation ability and transfection efficiency of micelle–DNA complexes (miceplexes) in the presence or absence of serum had been investigated on Hela and HepG2 cell lines. Uptake of micelles in Hela cells was faster and more effective than HepG2. After 1.5 hours, micelles were up-taken by Hela cells while no uptake was observed in HepG2 cells. Miceplexes showed lower transfection efficiency in both Hela and HepG2 cells grown in the serum free medium than chitosan polyplexes, with 7.25 × 10⁵ versus 1.47 × 10⁵ RLU.mg^-1 and 7.76 × 10⁵ versus 2.16 × 10⁵ RLU.mg^-1, respectively. Lower transfection efficiency of miceplexes could be attributed to their stronger complexation. Transfection in the presence of serum increased in both of the cell lines. These properties all together, make RA-chitosan micelles a potential gene carrier for active gene delivery into cells, particularly for transfection in the presence of serum.

Keywords

Retinoic acid-chitosan micelle

Miceplex

Non-viral gene carrier

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