Design of Intelligent Polymer of Gelatin- Poly N-isopropyla- crylamide under Gamma radiation for Cellular Applications

Saeed Heidari-Keshel; Bita Soleimani; Maryam Ebrahimi; Asghar Ashrafi Hafez; Razieh Fallahakbarpour; Mohammad Khaledian; Fatemeh Yousefi; Maryam Rostampour Kakroudi; Forough Fathi Arateh

Outlines

Abstract
Copyright

Design of Intelligent Polymer of Gelatin- Poly N-isopropyla- crylamide under Gamma radiation for Cellular Applications

Author(s):

Saeed Heidari-Keshel^{1, 2,*},

Bita Soleimani³,

Maryam Ebrahimi²,

Asghar Ashrafi Hafez⁴,

Razieh Fallahakbarpour⁵,

Mohammad Khaledian⁵,

Fatemeh Yousefi⁵,

Maryam Rostampour Kakroudi⁵,

Forough Fathi Arateh⁵

1Proteomics Research Center, Shahid Beheshti University of Medical sciences, Tehran, Iran

2Tissue Engineering Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

3Department of Chemistry, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

4Medical Education Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

5Department of Biomaterials Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 2, issue 2; 171-178

Published online:Oct 15, 2013

Article type:Research Article

Received:Sep 13, 2013

Accepted:Oct 09, 2013

How to Cite:Saeed Heidari-KeshelBita SoleimaniMaryam EbrahimiAsghar Ashrafi HafezRazieh FallahakbarpourMohammad KhaledianFatemeh YousefiMaryam Rostampour KakroudiForough Fathi Aratehet al.Design of Intelligent Polymer of Gelatin- Poly N-isopropyla- crylamide under Gamma radiation for Cellular Applications.J Rep Pharm Sci.2(2):e147751.

Abstract

Thermo-sensitive polymers were prepared by graft copolymerization of gelatin with N-Isopropylacrylamide via gamma radiation. Characterization of polymers such as DSC analysis, swelling in different ratios and cell assays were investigated. DSC and solubility analysis showed gelatin and N-Isopropylacrylamide monomers were grafted via gamma radiation successfully. Results show swelling of samples increased as gelatin increased. Swelling ratio and curves results administrated hyd-ro-philicity / hydrophobicity of hydrogel that this property is due to presence of NIsopropylacrylamide in different temperatures. The polymer was tested for harvesting epithelial cells after carrying out cell culture at 37 °C and incubating the confluent cells at 10°C for spontaneous detachment of cell sheet from polymer surface without enzyme treatment. These unique properties of the hydrogel would make it a promising support for drug delivery systems and tissue regeneration.

Keywords

Thermo- Sensitive Polymer

Gelatin

N-Isopropylacrylamide

Gamma Radiation

Epithelial Cell Sheet

comments