IJ Pharmaceutical Research
Synthesis and In Vitro Leishmanicidal Effects of Conformationally Restricted Analogues of Pentamidine
Farzin Hadizadeh1, 2,*, Mahmoud Reza Jaafari1, 2, Afshin Samiei2, Azam Mostafavirad1, Ebrahim Mohammadian2Abstract
Four conformationally restricted analogues of pentamidine were prepared. Then, different concentrations (0.039, 0.078, 0.156, 0.312 and 0.625 mg/mL) of each compound and two positive controls (amphotericin B and pentamidine, 0.625 mg/mL), one negative control (culture medium) and one solvent control (DMSO) were prepared and placed in 24-well plates containing 50000 parasite per well. Promastigotes of Leishmania major were incubated over a period of 2 days at 25°C; subsequently, percent of viable parasite in each well determined spectrophotometrically using MTT assay. The average EC50 for compounds 4a,b and 8a,b in DMSO was 0.098, 0.410, 0.150, 0.720 mg/mL, respectively. The average EC50 for positive controls pentamidine and amphotericin B was found to be 0.062 and 0.026 mg/mL. The control solvent had no significant effect on L. major promastigotes. All compounds had significant effect compared to DMSO and were less potent than positive controls.
Copyright
© 2022, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
In-vitro and In-vivo Antileishmanial Activity of a Compound Derived of Platinum, Oxaliplatin, against Leishmania Major
Ghasemi E, Ghaffarifar F, Dalimi A, Sadraei J. In-vitro and In-vivo Antileishmanial Activity of a Compound Derived of Platinum, Oxaliplatin, against Leishmania Major. Iran J Pharm Res. 2019;18(4):e126267. doi: https://doi.org/10.22037/ijpr.2019.15364.13046
In Vitro Effects of Ketotifen and Cromolyn Sodium on Promastigotes and Amastigotes of Leishmania major
Asgharpour Sarouey L, Khanaliha K, Rahimi-Moghaddam P, Khorrami S, Dayer MS, et al. In Vitro Effects of Ketotifen and Cromolyn Sodium on Promastigotes and Amastigotes of Leishmania major. Jundishapur J Microbiol. 2019;12(1):e82389. doi: https://doi.org/10.5812/jjm.82389
Antileishmanial Effects of Propolis Against Leishmania major In Vitro and In Vivo
Tavakoli P, Shaddel M, Yakhchali M, Raoufi N, Shamsi H, et al. Antileishmanial Effects of Propolis Against Leishmania major In Vitro and In Vivo. Ann Mil Health Sci Res. 2020;18(1):e100630. doi: https://doi.org/10.5812/amh.100630
Synthesis and In Vitro Anti-Leishmanial Evaluation of 1 -( 5 - Halo- 2 -Thienyl)- 2 -[5 -(5-Nitroheteroaryl)]-1 ,3 ,4 -Thiadiazol- 2 - Ylthio)Ethanone Derivatives
Adibi H, Foroumadi A, Heidari O, Aliabadi A, Kabudanian Ardestani S. Synthesis and In Vitro Anti-Leishmanial Evaluation of 1 -( 5 - Halo- 2 -Thienyl)- 2 -[5 -(5-Nitroheteroaryl)]-1 ,3 ,4 -Thiadiazol- 2 - Ylthio)Ethanone Derivatives. J Rep Pharm Sci. 2012;1(2):e147768. doi:
In Vitro and In Vivo Studies of Anti leishmanial Effect of Artemether on Leishmania infantum
Mortazavi Dehkordi N, Ghaffarifar F, Mohammad Hassan Z, Esavand Heydari F. In Vitro and In Vivo Studies of Anti leishmanial Effect of Artemether on Leishmania infantum. Jundishapur J Microbiol. 2013;6(5):e6379. doi: https://doi.org/10.5812/jjm.6379
Crossmark
Checking
- Scopus by DOI: 0
Last Update: 3 weeks ago
- Scopus by Title: 0
Last Update: 3 weeks ago
- Scopus by Title (Ref): 0
Last Update: 3 weeks ago
- CrossRef: 0
Last Update: 4 days ago
Ordering Reprints
Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC
Author(s):